Chatter, aka Venetian blinds: parallel lines in the tissue or separation of tissue into fine lines
Causes
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Remedies
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Locking mechanisms loose on microtome
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- Verify locking mechanisms are tight
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Dull blade
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- Move blade to an unused area or insert a new blade
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Blade angle too high
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- Reduce blade angle in 1-degree increments
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Sectioning too quickly
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- Slow down sectioning speed
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Poor tissue processing
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- Soak if over processed; reprocess if under processed
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